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Researchers discover what makes time-restricted dieting work

[Jan. 7, 2023: Shaunak Deota, Salk Institute]

Numerous studies have shown health benefits of time-restricted eating including increase in life span in laboratory studies. (CREDIT: Creative Commons)

Numerous studies have shown health benefits of time-restricted eating including increase in life span in laboratory studies, making practices like intermittent fasting a hot topic in the wellness industry. However, exactly how it affects the body on the molecular level, and how those changes interact across multiple organ systems, has not been well understood.

Time-restricted feeding or eating (TRF or TRE in humans) is a novel intervention in which nutrients are consumed within a consistent window of 8–10 h each day, resulting in pleiotropic health benefits that involve different tissues. Importantly, benefits are observed even when caloric intake or the type of diet remains unchanged, and qualitatively similar benefits are also observed in a number of human studies.


Benefits include improvements in glucoregulation, exercise capacity, endurance, motor coordination, sleep, blood pressure, liver triglycerides, plasma lipids, cardiac function, and gut health. Reductions in tumor growth, cancer risk, and the severity of neurodegenerative diseases have also been seen.

Now, Salk scientists show in mice how time-restricted eating influences gene expression across more than 22 regions of the body and brain. Gene expression is the process through which genes are activated and respond to their environment by creating proteins.


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The findings, published in Cell Metabolism have implications for a wide range of health conditions where time-restricted eating has shown potential benefits, including diabetes, heart disease, hypertension, and cancer.

“We found that there is a system-wide, molecular impact of time-restricted eating in mice,” says Professor Satchidananda Panda, senior author and holder of the Rita and Richard Atkinson Chair at Salk. “Our results open the door for looking more closely at how this nutritional intervention activates genes involved in specific diseases, such as cancer.


For the study, two groups of mice were fed the same high-calorie diet. One group was given free access to the food. The other group was restricted to eating within a feeding window of nine hours each day. After seven weeks, tissue samples were collected from 22 organ groups and the brain at different times of the day or night and analyzed for genetic changes.

Time-restricted eating reshapes gene expression throughout the body. In this illustration, the Ferris wheel displays the interconnected organ systems working smoothly during time-restricted eating, which is represented by the clock in the middle. (CREDIT: Salk Institute)

Samples included tissues from the liver, stomach, lungs, heart, adrenal gland, hypothalamus, different parts of the kidney and intestine, and different areas of the brain.

The authors found that 70 percent of mouse genes respond to time-restricted eating.


"By changing the timing of food, we were able to change the gene expression not just in the gut or in the liver, but also in thousands of genes in the brain,” says Panda.

Changing the quality or quantity of nutrient intake results in gene expression and functional changes that underlie observed health outcomes. (CREDIT: Cell Metabolism)

Nearly 40 percent of genes in the adrenal gland, hypothalamus, and pancreas were affected by time-restricted eating. These organs are important for hormonal regulation. Hormones coordinate functions in different parts of the body and brain, and hormonal imbalance is implicated in many diseases from diabetes to stress disorders. The results offer guidance to how time-restricted eating may help manage these diseases.


Satchidananda Panda

Interestingly, not all sections of the digestive tract were affected equally. While genes involved in the upper two portions of the small intestine—the duodenum and jejunum—were activated by time-restricted eating, the ileum, at the lower end of the small intestine, was not.

This finding could open a new line of research to study how jobs with shiftwork, which disrupts our 24-hour biological clock (called the circadian rhythm) impact digestive diseases and cancers. Previous research by Panda’s team showed that time-restricted eating improved the health of firefighters, who are typically shift workers.

The researchers also found that time-restricted eating aligned the circadian rhythms of multiple organs of the body.

“Circadian rhythms are everywhere in every cell,” says Panda. “We found that time-restricted eating synchronized the circadian rhythms to have two major waves: one during fasting, and another just after eating. We suspect this allows the body to coordinate different processes.”


Next, Panda’s team will take a closer look at the effects of time-restricted eating on specific conditions or systems implicated in the study, such as atherosclerosis, which is a hardening of the arteries that is often a precursor to heart disease and stroke, as well as chronic kidney disease.

Schematic diagram indicating the experimental setup for TRF intervention and sample collection strategy, along with a list of 22 collected tissues. (CREDIT: Cell Metabolism)

Other authors include Shaunak Deota, Terry Lin, April Williams, Hiep Le, Hugo Calligaro, Ramesh Ramasamy, and Ling Huang of Salk; and Amandine Chaix of the University of Utah.


The research was supported by the National Institutes of Health (grants CA258221, DK115214, CA014195, and AG065993) and the Wu Tsai Human Performance Alliance.

For more science news stories check out our New Discoveries section at The Brighter Side of News.


Note: Materials provided by Salk Institute. Content may be edited for style and length.


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